Activated Protein C (APC 99) Aptamer Details
ID# 7539
Structure
Chemistry
RNA
Target
Human Activated Protein C
Category
Protein
Affinity (Kd)
137 nM
Length
99
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprUprGp rAprGprAp rCprCprAp rGprCprCp rGprAprGp rUprGprGp rUprGprUp rCprUprGp rGprCprUp rAprUprUp rCprAprCp rUprGprGp rAprGprCp rGprUprGp rGprGprUp rGprGprAp rAprCprCp rCprCprUp rGprCprGp rCprAprCp rUprCprGp rUprUprUp rGprGprCp rUprGprUp rCprCprGp rGprGprCp rCprUprUp rCprGprGp rGprCprCp rGprGprGp rAprUprUp rAprUprCp rUprCprUp-3'
Molecular Weight: 31865.02
Extinction Coefficient: 931000
Binding Conditions / Buffer
Binding Buffer: 10 mM sodium citrate, 150 mM KCl (pH 7.6).
Incubation Time: 10 min.
Refolding Protocol: Heated at 70°C for 3 min then cooled at RT for 10 min.
Assay Type: Nitrocellulose filter.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Gal, S., et al. "Selection of a RNA aptamer that binds to human activated protein C and inhibits its protease function." European Journal of Biochemistry, 252(1998): 553-562.
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