Acetylcholine (ACh 6R) Aptamer Details
ID# 7807
Structure
Chemistry
DNA
Target
Acetylcholine
Category
Small Organic
Affinity (Kd)
N/A nM
Length
72
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCpdCpdGpdTpdCpdApdCpdApdCpdCpdTpdGpdCpdTpdCpdTpdCpdApdGpdGpdGpdGpdApdTpdCpdApdCpdApdTpdTpdCpdTpdTpdGpdApdCpdGpdGpdTpdGpdTpdGpdApdTpdApdCpdApdGpdTpdGpdCpdCpdTpdGpdGpdTpdGpdTpdTpdGpdGpdCpdTpdCpdCpdCpdGpdTpdApdTp-3'
Molecular Weight: 22123.35
Extinction Coefficient: 666100
Binding Conditions / Buffer
Binding Buffer: 0.5 M NaCl, 10 mM Tris HCl, and 1 mM MgCl2.
Incubation time: 1 hour
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
J.G. Bruno et al. Development of DNA aptamers for cytochemical detection of acetylcholine. In Vitro Cell. Dev. Biol.-Anim. 44(2008):63-72.
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