A5 Aptamer Details

ID# 8091

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Structure

Chemistry

DNA

Target

GLUT1 Glucose transporter

Sequence (3-char table code)

G A C U/T

5’-dTpdCpdGpdCpdApdCpdApdTpdTpdCpdCpdGpdCpdTpdTpdCpdTpdApdCpdCpdCpdApdTpdGpdGpdCpdTpdApdGpdGpdTpdGpdTpdTpdTpdApdTpdTpdApdApdTpdCpdCpdTpdGpdTpdApdGpdGpdApdTpdTpdTpdGpdCpdGpdGpdApdApdTpdCpdGpdTpdApdApdGpdTpdCpdCpdGpdTpdGpdTpdGpdTpdGpdCpdGpdApdAp -3'

Molecular Weight: 24647.9

Extinction Coefficient: 755700

Binding Conditions / Buffer

100 mg/mL of yeast tRNA (Thermo Fisher, AM7119) and 1 mg/mL of bovine serum albumin (RPI, A30075).

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Rosch, J.C., Neal, E.H., Balikov, D.A. et al. CRISPR-Mediated Isogenic Cell-SELEX Approach for Generating Highly Specific Aptamers Against Native Membrane Proteins. Cel. Mol. Bioeng. 13, 559–574 (2020). https://doi.org/10.1007/s12195-020-00651-y

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