A24-3 Aptamer Details
ID# 9049
Structure
Chemistry
DNA
Target
bPAG9
Category
Protein
Affinity (Kd)
2.02±0.28 nM
Length
56
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
dTpdTpdGpdApdApdGpdTpdGpdApdCpdGpdCpdCpdApdGpdGpdGpdTpdGpdGpdGpdGpdGpdGpdGpdTpdGpdGpdGpdTpdGpdTpdTpdGpdGpdCpdGpdTpdApdCpdApdCpdTpdTpdCpdGpdCpdApdTpdApdGpdCpdApdGpdGpdTp
Molecular Weight: 17544.3 g/mole
Extinction Coefficient: 541400
Binding Conditions / Buffer
The truncated aptamer was diluted to 2 μM in binding bufer and mixed with an equal volume of bPAG9 (fnal concentration, 1.5 μM), with binding bufer used as a control. Each
CD spectrum was obtained by accumulating two scans at
100 nm min⁻1with an instrument time constant of 1 s.
Data were collected from 220 to 350 nm at 1 nm intervals. The background spectrum (binding bufer) was subtracted from the CD data.
Binding Temp: 25 °C°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lu, C., Qin, J., Wu, S. et al. Structural optimization, characterization, and evaluation of binding mechanism of aptamers against bovine pregnancy-associated glycoproteins and their application in establishment of a colorimetric aptasensor using Fe-based metal-organic framework as peroxidase mimic tags. Microchim Acta 191, 713 (2024).
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