A23.2 Aptamer Details
ID# 7914
Structure
Chemistry
DNA
Target
Staphylococcus aureus enterotoxin A (SEA)
Category
Protein
Affinity (Kd)
235 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdApdTpdGpdApdTpdCpdGpdTpdApdGpdTpdCpdApdTpdTpdTpdApdApdApdApdTpdTpdTpdGpdApdApdTpdApdCpdTpdApdTpdCpdApdApdApdGpdTpdTpdApdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp
Molecular Weight: 24732.2
Extinction Coefficient: 804300
Binding Conditions / Buffer
20 mM Tris, 100 mM NaCl, 5 mM KCl, 1 mM CaCl2 and 1 mM MgCl2 ƒ?› 6H2O, pH 7.4
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Huang, Y. et al. "Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin A." Anal. Methods, 2014, 6, 690-697.
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