A2 Aptamer Details
ID# 8125
Structure
Chemistry
DNA
Target
M0-like and M2-like macrophages and monocytes
Category
Cells
Affinity (Kd)
20-45 nM
Length
52
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdApdApdGpdApdGpdTpdApdGpdApdTpdGpdApdApdApdCpdGpdTpdTpdTpdTpdTpdTpdCpdGpdCpdCpdCpdGpdApdTpdApdApdApdApdGpdGpdGpdApdCpdGpdTpdGpdCpdGpdTpdCpdApdGpdApdCpdAp-3'
Molecular Weight: 16149.5
Extinction Coefficient: 526500
Binding Conditions / Buffer
For individual aptamer binding, cells were incubated with aptamer that was in Wash Buffer supplemented with 10% FBS and 100 ug/mL tRNA.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Sylvestre, M.; Saxby, C.; Kacherovsky, N.; Gustafson, H.; Salipante, S.; Pun, S. Identification of a DNA Aptamer That Binds to Human Monocytes and Macrophages. Bioconjug. Chem. 2020 Aug 9; 31(8): 1899-1907. DOI: 10.1021/acs.bioconjchem.0c00247.
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