Aptagen

A10 Aptamer Details

ID# 8138
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Structure
A10 structure
Chemistry
DNA
Target
Bovine pregnancy-associated glycoproteins (bPAGs)
Category
Protein
Affinity (Kd)
11.7 nM
Length
56
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5’- dTpdTpdGpdApdApdGpdTpdGpdApdCpdTpdCpdCpdGpdCpdApdCpdTpdGpdGpdGpdTpdGpdGpdGpdTpdGpdGpdGpdApdGpdGpdGpdTpdCpdGpdTpdGpdCpdGpdGpdCpdTpdGpdGpdTpdCpdApdTpdApdGpdCpdApdGpdGpdGpdTp-3’
Molecular Weight: 17849.5
Extinction Coefficient: 545500

Binding Conditions / Buffer

150 mM NaCl, 20 mM HEPES, 1 mM KCl, 1 mM CaCl2, 1 mM MgCl2, pH 7.4
Binding Temp: 94°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Liu, C., Lu, C., & Shi, G. (2020). Selection, identification, and application of DNA aptamers against bovine pregnancy-associated glycoproteins 4. Analytical and Bioanalytical Chemistry. doi:10.1007/s00216-020-02666-w
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