A1 Aptamer Details
ID# 9075
Structure
Chemistry
DNA
Target
CD8+ T cell
Category
Protein
Affinity (Kd)
20.1 nM
Length
88
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdCpdGpdCpdApdGpdCpdApdCpdCpdCpdGpdTpdGpdGpdTpdApdGpdTpdGpdTpdApdTpdCpdApdGpdGpdGpdApdGpdApdCpdApdCpdTpdApdCpdGpdTpdGpdApdTpdGpdCpdApdGpdCpdTpdTpdGpdApdApdApdTpdGpdGpdApdCpdApdCpdGpdTpdGpdGpdCpdTpdTpdApdGpdTp
Molecular Weight: 27314.7 g/mole
Extinction Coefficient: 863600
Binding Conditions / Buffer
Na+
= 137mM; Mg++ = 5.5mM
Binding Temp: 4 °C°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kacherovsky N, Cardle II, Cheng EL, Yu JL, Baldwin ML, Salipante SJ, Jensen MC, Pun SH. Traceless aptamer-mediated isolation of CD8+ T cells for chimeric antigen receptor T-cell therapy. Nat Biomed Eng. 2019 Oct;3(10):783-795. doi: 10.1038/s41551-019-0411-6. Epub 2019 Jun 17. PMID: 31209354; PMCID: PMC6783348.
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