A07 Aptamer Details
ID# 9009
Structure
Chemistry
RNA
Target
TGFBR3
Category
Protein
Affinity (Kd)
2.47 nM
Length
95
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
rGprGprGprCprCprAprGprGprCprAprGprCprGprAprGprAprGprAprUprAprAprGprCprAprGprAprAprGprAprAprGprUprAprUprGprUprGprAprCprCprAprUprGprCprUprCprCprAprGprAprGprAprGprCprAprAprCprUprUprCprAprCprAprUprGprCprGprUprAprGprCprCprAprAprAprCprCprGprAprCprCprAprCprAprCprGprCprGprUprCprCprGprAprGprAp
Molecular Weight: 29246.8 g/mole
Extinction Coefficient: 931400
Binding Conditions / Buffer
For the first round of TECS-SELEX, the folded RNA was
transferred to two 100-mm-dishes with 70% confluent TbRIII
cells. After 10 min incubation at 37 °C, the RNA mixture was
removed, and the cell surface was washed twice with 2 ml of
buffer A, followed by the elution of the bound RNAs with 2 ml
of EDTA-containing buffer (PBS-based cell dissociation buffer, Gibco/Invitrogen) at 37 °C for 5 min.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ohuchi SP, Ohtsu T, Nakamura Y. Selection of RNA aptamers against recombinant transforming growth factor-beta type III receptor displayed on cell surface. Biochimie. 2006 Jul;88(7):897-904. doi: 10.1016/j.biochi.2006.02.004. Epub 2006 Mar 6. PMID: 16540230.
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