9R-e1 Aptamer Details

ID# 9645

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Structure

Chemistry

DNA

Target

5-HMF

Sequence (3-char table code)

G A C U/T

dGpdTpdApdGpdCpdGpdGpdCpdGpdGpdTpdGpdTpdGpdGpdGpdCpdApdTpdTpdTpdTpdGpdGpdGpdCpdTpdApdApdAp

Molecular Weight: 9374.1 g/mole

Extinction Coefficient: 290400

Binding Conditions / Buffer

The 9th library of aptamers were incubated with the target for 1 gr at 37C, then an enzyme called T5 Exo was added to the mixture to digest unbound ssDNA library.

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Liu, S., Cheng, J., Zhang, X., Qin, Y., Li, Y., Liu, H., Wu, Q., Hou, J., & Liu, X. (2025). T5 exonuclease improves the identification of aptamers specific for small molecules from the early screening library. Analytical Chemistry, 97(15), 8444–8451. https://doi.org/10.1021/acs.analchem.5c00251

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