8A-35 against Interleukin-8 (IL-8) Aptamer Details
ID# 9097
Structure
Chemistry
RNA
Target
interleukin-8
Category
Protein
Affinity (Kd)
0.00172 nM
Length
35
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprGprGprCprUprUprAprUprCprAprUprUprCprCprAprUprUprUprAprGprUprGprUprUprAprUprGprAprUprAprAprCprCp-3'
Molecular Weight: 11450.8 g/mole
Extinction Coefficient: 363500
Binding Conditions / Buffer
200 μl of binding buffer [30 mm Tris–HCl (pH 7.5), 150 mm NaCl, 1.5 mm MgCl2, 2 mm DTT, and 1% BSA] for 30 min at room temperature, with gentle shaking
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Sung HJ, Choi S, Lee JW, Ok CY, Bae YS, Kim YH, Lee W, Heo K, Kim IH. Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8. Biomaterials. 2014 Jan;35(1):578-89. doi: 10.1016/j.biomaterials.2013.09.107. Epub 2013 Oct 13. PMID: 24129312.
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