4520-8 Aptamer Details
ID# 8211
Structure
Chemistry
DNA
Target
Staphylococcal protein A
Category
Protein
Affinity (Kd)
0.22 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdCpdGpdGpdCpdTpdTpdCpdGpdGpdGpdTpdApdCpdCpdTpdApdTpdTpdApdTpdCpdGpdGpdTpdTpdTpdApdGpdCpdCpdCpdApdGpdTpdCpdApdGpdApdAp-3'
Molecular Weight: 12356.99
Extinction Coefficient:
Binding Conditions / Buffer
40 mmol l^−1 HEPES pH 7·5, 0·1 mol l^−1 NaCl, 5 mmol l^−1 KCl, 5 mmol l^−1 MgCl2 and 0.05% Tween-20.
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Baumstummler, A., Lehmann, D., Janjic, N., Ochsner, U.A., 2014. Specific capture and detection of staphylococcus aureus with high-affinity modified aptamers to cell surface components. Lett. Appl. Microbiol. 59, 422–431.
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