4-1BB ligand (M12-23) Aptamer Details
ID# 7724
Structure
Chemistry
2'-F-RNA
Target
4-1BB ligand on CD8+ T Cells
Category
Protein
Affinity (Kd)
40 nM
Length
96
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGp rAprGprAp rGprAprGp rGprAprAp rGprAprGp rGprGprAp fUprGprGp rGpfCprGprAp fCpfCprGp rAprApfCp rGpfUprGp fCpfCpfCp fUpfUpfCp rAprAprAp rGpfCpfCp rGpfUpfUp fCprApfCp fUprAprAp fCpfCprAp rGpfUprGp rGp fCprApfUp rAprApfCp fCpfCprAp rGprAprGp rGpfUpfCp rGprApfUp rAprGpfUp rApfCpfUp rGprGprAp fUpfCpfCp fCpfCpfCp fCp -3'
Molecular Weight: 31154.72
Extinction Coefficient: 952100
Binding Conditions / Buffer
Low Salt Binding Buffer: 20 mM Hepes, pH 7.4; 50 mM NaCl; 2 mM CaCl2 ;0.01% BSA.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
J. O. McNamara II et al.. Multivalent 4-1BB binding aptamers costimulate CD8+ T cells and inhibit tumor growth in mice. J. Clin. Invest. 118(2008):376-386.
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