2mHNE-5 Aptamer Details
ID# 8031
Structure
Chemistry
DNA
Target
HNE
Category
Protein
Affinity (Kd)
480 nM
Length
93
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
mCpmCpmCpmUpmGpmUpmUpmCpmGpmCpmUpmApmUpmCpmCpmCpmCpmCpmApmUpmCpmCpmCpmCpmCpmGpmApmUpmUpmGpmCp
Molecular Weight: 20649.39
Extinction Coefficient: 672360
Binding Conditions / Buffer
Binding BUffer (20mM HEPES, pH 7.5, 150mM NaCL, 6mM KCL, 2mM MgCl2)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Liu, Zhixia, et al. "Evolved polymerases facilitate selection of fully 2'-OMe-modified aptamers" Royal Society of Chemistry, 8 (2017): 8179-8182
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