20-5 Aptamer Details
ID# 8016
Structure
Chemistry
DNA
Target
E. coli
Category
Cells
Affinity (Kd)
3.9 nM
Length
45
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdTpdTpdCpdGpdCpdCpdCpdCpdCpdGpdTpdGpdTpdTpdCpdCpdGpdApdCpdTpdGpdGpdTpdApdTpdCpdTpdTpdCpdApdCpdGpdTpdCpdTpdTpdCpdGpdApdGpdTpdGpdTp-3'
Molecular Weight: 13711.9
Extinction Coefficient: 403300
Binding Conditions / Buffer
1X PBS, 0.1 mg mL-1 salmon sperm, 1% bovine serum albumin, and 0.05% Tween-20
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kim HR, Song MY, Chan Kim B. Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method. Analytical Biochemistry. 2020 Feb;591:113542. DOI: 10.1016/j.ab.2019.113542.
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