2′-O-Methyl modified RNA aptamer Aptamer Details
ID# 7874
Structure
Chemistry
DNA
Target
p53
Category
Protein
Affinity (Kd)
N/A nM
Length
75
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-mGpmGpmGpmCpmGpmApmApmUpmUpmCpmGpmGpmGpmUpmUpmGpmGpmApmUpmApmGpmUpmApmGpmGpmCpmGpmCpmApmUpmApmUpmGpmGpmCpmApmUpmCpmUpmUpmCpmGpmUpmGpmGpmUpmUpmGpmUpmGpmUpmApmUpmUpmGpmCpmCpmCpmUpmUpmUpmApmGpmUpmGpmApmGpmGpmGpmUpmUpmApmApmUpmUp-3'
Molecular Weight: 25257.7
Extinction Coefficient: 753400
Binding Conditions / Buffer
0.15 mM Hepes [pH 7.4], 0.3 mM KCl, 6 uM dithiothreitol [DTT], 6 uM MgCl2, 1.1 uM ethylenediaminetetraacetic acid [EDTA], 6% glycerol, and 3 uM phenylmethanesulfonyl fluoride [PMSF]
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Shwetha, N. et al. "Aptamerƒ??nanoparticle-based chemiluminescence for p53 protein." Analytical Biochemistry 2013, 441: 73-79.
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