2-17-2 Aptamer Details

ID# 8236

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Structure

Chemistry

DNA

Target

E. coli

Sequence (3-char table code)

G A C U/T

5'-dTpdCpdApdApdGpdGpdTpdCpdGpdCpdApdApdTpdTpdTpdCpdGpdApdGpdCpdApdGpdApdApdCpdTpdTpdApdApdGpdAp-3'

Molecular Weight:

Extinction Coefficient:

Binding Conditions / Buffer

This work incubated 10^5 CFU (colony forming units) and the library in 20 mm Tris HCl, 5 mM KCl, 100 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 0.02% Tween-20 for 1 hour at 37°C according to reference 21 (see below) of this entry's literature. Kim, H. R.; Song, M. Y.; Kim, B. C. Rapid isolation of bacteria specific aptamers with a non-SELEX-based method. Anal. Biochem. 2020, 591, No. 113542. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841571/

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Mengyue Liu, Lingjun Geng, Fengjuan Zhang, Shouyi Dou, Falan Li, Zhanli Liu, Yemin Guo, and Xia Sun Journal of Agricultural and Food Chemistry 2022 70 (50), 15990-15998 DOI: 10.1021/acs.jafc.2c06167 https://pubs.acs.org/doi/abs/10.1021/acs.jafc.2c06167?ref=PDF

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