1G8-14 Aptamer Details
ID# 7934
Structure
Chemistry
RNA
Target
Ebola virus inhibitory domain (eVP35 IID) (EBOV) (eVP35)
Category
Protein
Affinity (Kd)
3.7 nM
Length
70
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
rGprGprGprAprGprAprCprAprAprGprAprApUrAprAprAprCprGprCpUrCprAprAprGprGprCprApUUUrCpUrGprCpUrAprGpUrCpUrGprGpUUrGpUrAprAprGprApUrApUUrCprAprAprCprAprCprGpUrGprAprGpUUUrCprGprAprCprAprGprGprAprGprGprCpUrCprAprCprAprAprCprAprGprGprCp
Molecular Weight: 22934.2
Extinction Coefficient: 715100
Binding Conditions / Buffer
10mM HEPES (pH 7.0), 150mM NaCl, 2mM TCEP (tris(2-carboxyethyl)phosphine), 1mM MgCl2
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Jennifer M. Binning, Tianjiao Wang, Priya Luthra, Reed S. Shabman, Dominika M. Borek, Gai Liu, Wei Xu, Daisy W. Leung, Christopher F. Basler, and Gaya K. Amarasinghe, ƒ??Development of RNA aptamers targeting ebolavirus VP35ƒ? Biochemistry 2013, 52, 8406-8419.
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