1G8-14 Aptamer Details
ID# 7947
Structure
Chemistry
RNA
Target
Ebola Virus VP35
Category
Protein
Affinity (Kd)
3.7 nM
Length
90
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
rGprGprGprAprGprAprCprAprAprGprAprAprUprAprAprAprCprGprCprUprCprAprAprGprGprCprAprUprUprUprCprUprGprCprUprAprGprUprCprUprGprGprUprUprGprUprAprAprGprAprUprAprUprUprCprAprAprCprAprCprGprUprGprAprGprUprUprUprCprGprAprCprAprGprGprAprGprGprCprUprCprAprCprAprAprCprAprGprGprCp
Molecular Weight: 29057.6
Extinction Coefficient: 914300
Binding Conditions / Buffer
10 mM HEPES (pH 7.0), 150 mM NaCl, 2mM TCEP (tris(2-carboxyethyl)phosphine), and 1 mM MgCl2 and filtered through nitrocellulose membrane
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Binning, J., Wang, T., Luthra, P., et al. (2013) Development of RNA aptamers targeting Ebola Virus VP35. Biochemistry, 52(47):8406-8419. doi: 10.1021/bi400704d
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