14-16 Aptamer Details

ID# 7865

Back to Search Results

Structure

Chemistry

2'-F-RNA

Target

Mouse P68 RNA Helicase (Ddx5)

Sequence (3-char table code)

G A C U/T

5'-rGprGprGp rAprGprGp rApfCprGp rApfUprGp fCprGprGp fCprAprGp fUprGpfCp fCpfCprAp rApfCpfCp rGprGprAp rApfCprAp rApfCprAp rApfCpfCp rApfCpfCp rGprGpfCp rGprGpfCp fUpfCpfCp fUprGpfCp fUpfCprAp rGprApfCp rGprApfCp fUpfCprGp fCpfUprGp rAprGprGp rApfUpfCp fCprGprAp rGprAp-3'

Molecular Weight: 25949.66

Extinction Coefficient: 769500

Binding Conditions / Buffer

For selection: 5 nmol of aptamer pool in 200 µl of PBS, injected systemically and allowed to circulate for 20 minutes For binding assay: 10 mM Tris-HCl (pH 7.0), 150 mM NaCl, 10% (v/v) glycerol, 2.5 µg/ml tRNA. 10 minute incubation at 37°C For affinity purification: 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, 0.1% BSA, 0.1 µM tRNA at 4°C for 2 hours

Binding Temp: 37°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Mi et al. ƒ??In vivo selection of tumor-targeting RNA motifsƒ?. Nature Chemical Biology 1(2010): 22-24. DOI: 10.1038/NCHEMBIO.277

Request a Quote Have your aptamer oligo synthesized by Aptagen.