1.5 M Short Anti-Heparanase Aptamer Aptamer Details
ID# 7956
Structure
Chemistry
DNA
Target
Heparanase
Category
Protein
Affinity (Kd)
n/a nM
Length
30
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdCpdTpdTpdTpdTpdGpdApdApdTpdGpdTpdGpdGpdCpdApdApdCpdApdApdApdTpdTpdCpdGpdApdCpdApdGpdGp-3'
Molecular Weight: 9254.11
Extinction Coefficient: 298000
Binding Conditions / Buffer
Cell Culture Medium: 50% DMEM, 50% Hamƒ??s F-12 Cell culture medium supplemented with: 50 mg/ml ascorbic acid, 250 ng/ml amphotericin B, 5mg/ml insulin (bovine pancreas), 0.4 ng/ml hydrocortisone and 10% heat-inactivated fetal bovine serum.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Simmons SC, Jamsa H, Silva D, Cortez CM, McKenzie EA, et al. (2014) Anti-Heparanase Aptamers as Potential Diagnostic and Therapeutic Agents for Oral Cancer. PLoS ONE 9(10): e96846. doi:10.1371/journal.pone.0096846
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